Aim of the present study to the synthesis of different piperazine derivatives by using different aldehydes. Anti-oxidant contains vitamin E, C, A and Beta – carotene. They are present in some fruits, vegetables, fixed oils and fish. The Anti-oxidant present in them act by one of two mechanisms. They either prevent the synthesis of oxygen-free radicals or have scavenging effect to trap this radicals. Structure of the synthesized compounds will be confirmed by spectral analysis like IR, NMR and Mass spectrum. The synthesized compounds will be subjected for Anti-oxidant evaluation.

Urinary tract infections (UTI) are among the most common bacterial infections in humans. Asymptomatic bacteriuria has been observed to be a strong criterion for urinary tract infections; it is therefore a means of predicting UTI. In this study, we investigated the prevalence of asymptomatic bacteriuria among female students of Imo State University Owerri (IMSU), a tertiary institution in southeast Nigeria. Mid stream urine specimens were collected from 253 apparently healthy asymptomatic undergraduate female students of IMSU between ages of 17 and 26 who were randomly sampled. Each specimen was cultured on CLED and MacConkey agar, incubated overnight at 370C. Bacteria isolates were characterized and identified based on conventional microbiological techniques. Antibiotic susceptibility tests were performed using Kirby-Bauer’s disc diffusion method according to performance standards of Clinical Laboratory Standard Institute guidelines. The results showed that, the total prevalence rate of bacteriuria was 13.8% with the highest rate occurring in students within age group of 20-22 (8.3%). Out of the 253 urine specimens cultured, a total of 35 bacteria isolates were identified comprising Escherichia coli (37.9%), Staphylococcus aureus (20.7%), Klebsiella pneumonia (17.2%), Pseudomonas aeruginosa (13.8%) and Proteus mirabilis (10.3%). All isolates tested were 100% susceptible to ciprofloxacin, ofloxacin and gentamicin but showed 100% resistance to amoxycillin and cotrimaoxazole. Proteus mirabilis were 100% sensitive to all antibiotics except cotrimaoxazole and amoxycillin. Staphylococcus aureus and Escherichia coli exhibited multidrug resistance to antibiotics belonging to ≥ 4 antibiotic classes. Escherichia coli were the most common bacteria for asymptomatic bacteriuria in female university undergraduates in this study. Statistical analysis showed that bacteriuria rate among the students residing in different hostels was not significantly different in any of the sampled hostels P<0.05. The incidence of the bacteriuria in a healthy population is of significant public health concern, thus, education and awareness on the need for proper personal hygiene should be encouraged.

Indigenous medicines for their known least side effects than allopathic medicine have been investigated for unsolved problems in diseases like Cancer, AIDS. This study aims to evaluate the Anti-tumour activity of various extracts of leafs of Vitex leucoxylon by invitro method using MTT assay method. These extracts were screened for its cytotoxicity against HeLa cell lines at different concentrations to determine the IC 50 (50% growth inhibition). Ethanol and Chloroform extracts showed more significant effect on the HeLa cell line when compared to Pet. ether extract.