A highly sensitive isocratic reverse phase high performance liquid chromatographic method was developed and validated for the estimation of Metformin and Repaglinide in Bulk and tablet dosage forms. Separation of metformin and Repaglinide successfully achieved on Hypersil, C18, v size 250mm 4.6mm column or equivalent utilizing methanol buffer pH 6.0 with OPA (40:60)v/v as mobile phase at a flow rate of 1.0ml/min and the eluates was monitored at 242 nm. Chromatogram showed peak at a retention time of 3.60 min and 2.60 min. The method was validated for system suitability, linearity, precision, accuracy, specificity, ruggedness, robustness, LOD and LOQ. Recovery of metformin and Repaglinide were found to be in the range of 99.76% and 100.2833% and showing linearity in the range of 100-1000µg, ml and 0.5-2 µg, ml. Proposed method can be successfully applied for the quantitative determination of metformin and Repaglinide in Bulk and tablet dosage form.

Transdermal drug delivery systems is a new approach to provide prolonged action of the drug. Recently niosome are becoming popular in the field of topical drug delivery. This niosome formulation is generally accepted by the patient due to non-toxic and non - immunogenic. Niosome are the microscopic lamellar structures composed of non-ionic surfactants and cholesterol. It includes various types of non-ionic surfactants like ester linked surfactants, alkyl ethers, tweens and spans. Niosome can be prepared by different methods includes thin film hydration, either injection emulsion method etc. The niosome gel can be prepared by using 1% carbopol gel. The prepared formulation can be evaluated for size, vesicular diameter, entrapment efficiency, in vitro release. Niosome can be enhanced by developing noisome gel formulation for prolonged drug release pattern. Finally this review presents that, the Tran dermal delivery via niosome gel is a suitable delivery for a better activity of drug for a long period of time.

Pippali (Piper longum) belongs to Piperaceae, cultivated for its fruit widely used medicine for diseases of the respiratory tract, viz. cough, bronchitis, asthma, etc. as counter- irritant, analgesic and as anti-inflammation. Here in we studied the antibacterial property of Piper longum protein isolated from hot water extract of Piper longum. The protein was isolated from Piper longum by 65% ammonium sulphate precipitate and followed by dialysis using molecular cutoff membrane. The proteins were examined for their antioxidant and antibacterial activity. The protein at 25 g concentration showed about 59% inhibition against DPPH. The proteins were checked for their antibacterial activity against human pathogenic bacteria’s like Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas, Salmonella typhimurium, Streptococcus Sps, Staphylococcus aureus, Vibrio cholera. MIC value of antioxidant protein ranged from 90-106 µg/ml. MIC was about 12-15 µg/ml. Streptomycin was used as a standard antibiotic. These results show that, the antioxidant protein from Turmeric is an effective antioxidant as well as antimicrobial agent.